Kansas
This program is funded by the National Science Foundation
grant number PHY-0851599
Research in Atomic Force
Microscopy
to stretch single protein molecules
Katarzyna Malek (Jagiellonian
University, Krakow, Poland)
e-mail: kadiabatam at gmail.com
supervisor: Prof. Robert Szoszkiewicz
e-mail: rs at phys.ksu.edu
Welcome to my
webpage. This page summarizes my experience doing research for the Summer 2011 in the Szosz-lab!
Project Goals: Describle
a mechanical properties of proteins (I274 and NOTCH) using Single
Molecule Force Spectroscopy (SMFS). Stretch single protein molecules using Force-Extension (FX SMFS) and Force-Clamp (FC SMFS).
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Obteined protein
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Protein purification
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Polyacrylamide gels
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Checking Protein Folding
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Stretched single
protein molecules
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Obtained
protein (I274 and NOTCH) bacteria cells: Roseta-gami2(DE3)pLysS ,
XL10GOLD; plasmid: pQE30L, derived from T5lac promoter system
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Inoculated agar plates, pre-culture, large
scale expression, prepared cells extract, sonication
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Protein purification on Co resin HisTALON superflow (for I274) and Ni resin (prefer for
NOTCH)
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Made polyacrylamide gels for protein,
electrophoresis
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Measured a concentrate of protein on spectrometer
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Made evaporated gold substrates
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Checking Protein Folding in a bulk: Circular Dichroism
Spectroscopy, Fluorescence Spectroscopy
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Stretched single
protein molecules using Single
Molecule Force Spectroscopy (SMFS).
Final
Presentation: Download the presentation in pdf format.
Lectures: Dr. Weaver gave very interesting lectures, which were extremely useful in learning the physics to understand the other student’s projects.
Ethics Class: Discussion during ethics class were very noteworthy. I knew some basic ethical theory.
Discussions about fraud, dishonesty, authorship, plagiarism were very
interesting and didactic.
About Me: I am a student of the 2nd level undergraduate program for major in biophysics at the Faculty of Physics, Astronomy, and Applied Computer Science of the Jagiellonian University in Krakow, Poland. The studies allow me to extend the knowledge in the field of biophysics acquired previously at the 3 year studies of the 1st level undergraduate program. Especially, I am interested in applied applications of physics methods in Medicine, and Biology. I think that worked in the Szosz-Lab of the Kansas State University allowed me to acquired new skills and experience in AFM technique. In my opinion took part in REU activities had a great impact on my education and development of necessary skills allowed for co-operative research in cutting edge science and in the international environment.
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Szosz-lab at KSU
Department of Physics – laboratory where I worked this summer on
that project
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K-State Physics REU - Research Experience for Undergraduates
program
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Department of Physics of Nanostructures
and Nanotechnology at Jagiellonian University- my
home group website
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Kobior –
my home city