Dr. Matthew Antonik

University of Kansas

Monday, December 1, 2008

4:00 p.m.

Cardwell 102

Single Molecule Biophysics: Fluorescence Measurements of the Dynamics, Flexibility and Heterogeneity of Large Biomolecules

Fluorescence measurements have revealed much about the structure and workings of biological systems. Recent quantitative advancements in the analysis of the fluorescence signals allow subtle heterogeneities, molecular flexibility, and dynamics of biological systems to be extracted from the data. The use of these techniques to extract information about the biophysics of a biomolecular system will be presented. The potential for extending the analysis to multiple fluorophores and the use of model molecules as probes for more complex systems will be discussed.